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Legumes heat treatment

Good day professional, I'm trying to treat legumes through freeze drying, due to off-flavors, bad odours cause by the presence of certain enzymes.

My question:

Do anybody has any idea of how I can achieve a low pressure manually in my lab.

2. Do anybody has any other effective techniques of treating plant protein

asked by @iyanuakande • 1/23/2021

Answers (23)

asked by @roy • 1/23/2021
21

LYOphillization or freeze drying does not deactivate
lipoxygenase enzyme responsible for legume taste., Germination is better...
Pressure cooking beans is one way to reduce beans taste..Often most kitchens have a pressure cooker.Anothee is soaking in 0.5% NaHCO3 solution fir at least 6 hours
You combine both methods you get much better result.

@roy • 1/23/2021
30

Thanks Roy, what was on my mind was to dehull the legume and or heat treat for 121 for 10min to avoid denaturation and finish the process with lypholisation.

Is it possible?

121 attack the enzymes

@iyanuakande • 1/23/2021
3

But hope the pressure cooking won't denature the protein... will like to use it for plant milk

@iyanuakande • 1/23/2021
19

Dehulling does not remove lipoxygenase enzyme. heat treatment might be but the taste due enzymatic reaction is not driven off.Pressure cooking where steam drives of volatile might help..

@roy • 1/23/2021
19

Yes I know but the water during dehulling initiate enzymatic reaction

@iyanuakande • 1/23/2021
4

Thank you for that contribution

@iyanuakande • 1/23/2021
15

If dehulling initiate lipoxygenase action then you should presoak the beans In NaHCO 3 solutiin after being dehulled to curtail it.
Why do you want to lyopphillize it.if its not effective?

@roy • 1/23/2021
22

I want to dry it after heat treatment while maintaining the color

@iyanuakande • 1/23/2021
11

You want to retain color then inactivating the chlorophhylase, catalase- peroxidase enzyme by heat treatment will do it, but to drive off traces of lipoxygenase reaction you need to steam it off.

@roy • 1/23/2021
17

[quote="iyanuakande, post:4, topic:8470, full:true"]
But hope the pressure cooking won't denature the protein... will like to use it for plant milk
[/quote]

FOR example if you want to make soy milk it needs heat treatment to inactivate enzymes ,as well as kill pathogens , denature proteins to make it more digestible further destroying the antinutrient factor called Lectins.

@roy • 1/23/2021
12

Are you saying the protein needs to be denature to make it readily available and absorbable

@iyanuakande • 1/24/2021
17

Yes, the same principle why you cook meat and even legumes; the latter contain lectins which affect nutrients absorption..

@roy • 1/24/2021
18

But it will reduce it functionality in the Application at long run... I'll try it...

Then Roy, can you advice on how I can steam off the volatile.. what process would advice

@iyanuakande • 1/24/2021
26

What if I connect the vacuum to the pressure pot

@iyanuakande • 1/24/2021
28

[quote="iyanuakande, post:14, topic:8470, full:true"]
Then Roy, can you advice on how I can steam off the volatile.. what process would advice
[/quote]

Pressure cooking foods releases excess steam from the pressure valve so,off goes with it the volatiles

@roy • 1/24/2021
26

Good morning Roy, so after the pressure cooking, how can I dry it.

@iyanuakande • 1/25/2021
27

Can you guide me with a simple flow chart

@iyanuakande • 1/25/2021
12

Lol..that is kitchen work....if you know how to cook you don't need flowchart...🤣

@roy • 1/25/2021
20

There is nothing complicated in making soy milk, if thats a similar goal with your legume milk.You already did the groundwork..You are only stymied by the off flavor due to the lipoxygenase and color change due chlorophyllase / catalase / peroxidase enzymes I mentioned. If you had succeeded in it then drying would not be difficult in what ever means you had in mind.FYI, your method is complex using lyophilizarion etc due to the lack of understanding of the mechanism behind your project .
Now that you are already enlightened after a series 9f discussion, the work actually becomes easier.....

@roy • 1/25/2021
4

No Roy, would like to know the suggested temperature and time for pressure cooking, then I will dry from that hence

@iyanuakande • 1/25/2021
5

I'm as much sensitive to the protein denaturation. I working towards a functional plant protein powder at long run

@iyanuakande • 1/25/2021
28

@iyanuakande
Well you worry to much about protein denaturation but its part of the process to ensure that your protein will have better digestibility..
Regarding the processing parameters you have to establish that yourself as thats your experiment.
R& D work is not about asking for every morsel of idea from other people;rather you have to know what you are doing, You have to understand the molecular side ,the mechanism, and the reason why you are doing such thing Before embarking on a project you had done much thought and information search and other ground work.
In your case you should have studied the chemistry of the material you are using .If there are some small gaps of knowledge this group can provide suggestions.
But if you demand everything to be spoon fed to you, then you are losing the value of the project you are supposed to be proud to pursue .
I have given you pointers and ,ideas but it appears it all confounds you meaning, you got lost in your own project.
If thats the case my series of suggestions did not help you at all.. 🙂

@roy • 1/25/2021
29

I'm trying to get the direction of your query.

What would be the form and use of the product after treatment? I think this would guide the treatment type to applied.

@anon64889931 • 3/22/2021